Isolation and characterisation of 11 microsatellite loci in the Ecuadorian Hillstar Oreotrochilus chimborazo

Doi: http://dx.doi.org/10.13157/arla.64.1.2017.sc3

Authors: Nora H. OLEAS, Nick HARVEY, Carlos A. RODRÍGUEZ-SALTOS and Elisa BONACCORSO

E-mail: noraoleas@gmail.com

Published: Volume 64.1, January 2017. Pages 85-89.

Language: English

Keywords: Andes, Ecuadorian Hillstar, hummingbird, microsatellite and Paramo

Summary:

The Ecuadorian Hillstar Oreotrochilus chimborazo is a high Andean hummingbird that inhabits paramo ecosystems from extreme southern Colombia to southern Ecuador. The species is currently divided into three subspecies that differ in morphology and geographic distribution but no molecular differences have been found between them using mitochondrial markers. The aim of this study was to isolate and test microsatellite loci to further explore possible genetic variability between and within these subspecies. Screening an enriched genomic library of O. chimborazo we obtained eleven polymorphic microsatellite loci. These markers will be used to further elucidate evolutionary patterns in the species.

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